Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Landais, S.; Gounon, P.; Laurent-Winter, C.; Mazie, J.C.; Danchin, A.; Barzu, O.; Sakamoto, H.
    Immunochemical analysis of UMP kinase from Escherichia coli (1999), J. Bacteriol., 181, 833-840.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.22 expression in Escherichia coli BL21(DE3)/pDIA17 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.22 D174N the enzyme is not recognized by a monoclonal antibody Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.22 cytoplasm near the bacterial membranes Escherichia coli 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.22 26000
-
 x * 26000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.22 ATP + UMP Escherichia coli
-
 ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.22 Escherichia coli P0A7E9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.22
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.22 4°C, 50 mM Tris-HCl (pH 7.4) as insoluble proteins, after solubilization with 0.1 M borate (pH 9) or with 1 mM UTP in 50 mM Tris-HCl (pH 7.4), the enzyme is fully active Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.22 ATP + UMP
-
 Escherichia coli ADP + UDP
-
 ?
2.7.4.22 ATP + UMP 30°C, pH 7.4 Escherichia coli ADP + UDP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.22 ? x * 26000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.4.22 UMP kinase
-
 Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.22 Escherichia coli isoelectric focusing
-
 7.24